The Greek mezzo-soprano, Diana Eustrati, studied singing at the Royal College of Music in Athens.
Diana Eustrati made her operatic debut at the Royal Opera House, Athens in 1940, remaining there until 1948. She sang at the Berlin Comic Opera for many years and between 1950 and 1959 was a member of the Berlin State Opera. Eustrati was well-known to European audiences, appearing in recitals and concerts in Cologne, Frankfurt, London and at the International Händel Festival, Göttingen. Her operatic roles included Carmen, Eboli in Don Carlo, Leonora in La Favorita, Dalila in Samson and Dalila, Azucena in Il Trovatore, Meg Page in Falstaff, Fricka in the Der Ring des Nibelungen, and Octavian in Rosenkavalier. Eustrati appeared in the premiere of Paul Dessau's The Trial of Lukullus at the Berlin State Opera in 1951. Important conductors with whom Eustrati worked included Abendroth, Fritz Lehmann and Fricsay.
Diana Eustrati's recordings appear on the Ariola, Eterna, Royale, Supraphon, HMV, Discophile Français and DGG labels.